G6171
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 | Immunofluorescence Rat cerebellum sections ethanol fixed and paraffin embedded were stained with 2.5-5 μg /mL of Monoclonal Anti-GFAP (Cat. No. G6171). The antibody was developed with Goat Anti-Mouse IgG, FITC conjugate (Cat. No. F8771). |
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G6171
Sigma
Monoclonal Anti-Glial Fibrillary Acidic Protein antibody produced in mouse
~1.0 mg/mL, clone G-A-5, purified immunoglobulin, buffered aqueous solution
| Synonym: | Anti-GFAP |
Description
| Immunogen | purified GFAP from pig spinal cord. |
| Specificity | The antibody does not cross react with vimentin, which is frequently coexpressed in glioma cells and some astrocytes. |
| Physical form | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
Properties
| antibody form | purified immunoglobulin |
| clone | G-A-5 (monoclonal) |
| form | buffered aqueous solution |
| mol wt | antigen mol wt ~50 kDa |
| species reactivity | rat, pig, human |
| concentration | ~1.0 mg/mL |
| application(s) | immunoblotting: suitable |
| immunocytochemistry: suitable | |
| immunohistochemistry: suitable | |
| indirect immunofluorescence: 2.5-5 μg/mL using alcohol-fixed sections of rat brain/cerebellum. | |
| isotype | IgG1 |
| Gene Information | human ... GFAP(2670) |
| rat ... Gfap(24387) | |
| mouse ... Gfap(14580) | |
| shipped in | dry ice |
| storage temp. | −20°C |
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| Array | XP725, Panorama® Antibody Microarray - XPRESS Profiler725 Kit |
